Caballero Santiago, Guix Susana, El-Senousy Waled Morsy, Calicó Ignasi, Pintó Rosa M, Bosch Albert
Enteric Virus Group, Department of Microbiology, University of Barcelona, Barcelona, Spain.
J Med Virol. 2003 Oct;71(2):245-50. doi: 10.1002/jmv.10476.
The relationship between cases of persistent diarrhoea and the levels and type of human astrovirus was investigated. The potential correlation between human astrovirus excretion levels and the occurrence of protracted gastroenteritis was elucidated after quantifying astroviruses in faecal samples by a competitive RT-PCR. This assay was developed employing an internal RNA standard constructed for this purpose and showed a threshold of positivity of 3.4 x 10(4) genomes per gram of faeces. By this procedure, the levels of astrovirus, belonging to serotypes 1, 2, 3, 4, and 8, in faecal samples could be ascertained to range from 3.4 x 10(8) to 1 x 10(13) per gram of faeces. The mean viral titre in the serotype 3-containing faeces was higher than in any of the other serotype-containing samples. In children with no background disease, persistent gastroenteritis cases were detected in 8.5% of the astrovirus infections, and 37.5% of those were associated with astrovirus type 3 infection. In addition, 42.9% of astrovirus 3 isolates were implicated with persistent cases, some of them lasting for 3 months. Other type 3 isolates, detected in the faeces in very large numbers, caused severe gastroenteritis.
研究了持续性腹泻病例与人类星状病毒水平及类型之间的关系。通过竞争性逆转录聚合酶链反应（RT-PCR）对粪便样本中的星状病毒进行定量后，阐明了人类星状病毒排泄水平与迁延性胃肠炎发生之间的潜在相关性。该检测方法是利用为此目的构建的内部RNA标准开发的，显示每克粪便的阳性阈值为3.4×10⁴个基因组。通过该程序，可以确定粪便样本中属于1、2、3、4和8型血清型的星状病毒水平为每克粪便3.4×10⁸至1×10¹³个。含3型血清型粪便中的平均病毒滴度高于任何其他含血清型样本。在无基础疾病的儿童中，8.5%的星状病毒感染检测到持续性胃肠炎病例，其中37.5%与3型星状病毒感染有关。此外，42.9%的3型星状病毒分离株与持续性病例有关，其中一些持续3个月。在粪便中大量检测到的其他3型分离株引起严重胃肠炎。
