Wang Xinyue, Ma Tong, Chen Zhiyuan, Liu Yang, Wang Kexin, Liu Guangxiu, Li Kesheng, Chen Tuo, Zhang Gaosen, Zhang Wei, Zhang Binglin
Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China.
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, China.
Viruses. 2025 Jan 11;17(1):86. doi: 10.3390/v17010086.
Recent decades have seen growing attention on viruses in the environment and their potential impacts as a result of global epidemics. Due to the diversity of viral species along with the complexity of environmental and host factors, virus extraction and detection methods have become key for the study of virus ecology. This review systematically summarises the methods for extracting and detecting pathogens from different environmental samples (e.g., soil, water, faeces, air) and biological samples (e.g., plants, animals) in existing studies, comparing their similarities and differences, applicability, as well as the advantages and disadvantages of each method. Additionally, this review discusses future directions for research in this field. The aim is to provide a theoretical foundation and technical reference for virus ecology research, facilitating further exploration and applications in this field.
近几十年来，由于全球流行病的影响，环境中的病毒及其潜在影响受到了越来越多的关注。由于病毒种类的多样性以及环境和宿主因素的复杂性，病毒提取和检测方法已成为病毒生态学研究的关键。本综述系统地总结了现有研究中从不同环境样本（如土壤、水、粪便、空气）和生物样本（如植物、动物）中提取和检测病原体的方法，比较了它们的异同、适用性以及每种方法的优缺点。此外，本综述还讨论了该领域未来的研究方向。目的是为病毒生态学研究提供理论基础和技术参考，促进该领域的进一步探索和应用。
