Deng A, Yang X, Wu C, Yao L, Li Y
Department of Nephrology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.
J Tongji Med Univ. 1999;19(4):307-9. doi: 10.1007/BF02886970.
To elucidate the pathogenesis of acute renal failure (ARF) with nephropathia epidemic (NE), provide experimental evidence for the new therapy to NE and observe the effects of Arg-Gly-Asp (RGD) peptides on adhesion of renal tubular epithelial cell (RTEC), urine specimens of patients were collected under sterile conditions. Detached RTECs were separated, cultured and identified. Hantan Virus antigen was determined by using indirect immunofluorescence method and effects of RGD on adhesion of RTECs was observed by subgroup counting as well as by flow cytometry. This study showed that: (1) sublethal RTECs existed in the urine of NE-ARF patients, which could be cultured in monolayer form; (2) there was NE antigen in RTECs; and (3) adhesion of RTECs could be inhibited by RGD.
为阐明流行性肾病（NE）并发急性肾衰竭（ARF）的发病机制，为NE的新疗法提供实验依据，并观察精氨酸-甘氨酸-天冬氨酸（RGD）肽对肾小管上皮细胞（RTEC）黏附的影响，在无菌条件下收集患者尿液标本。分离、培养并鉴定解离的RTEC。采用间接免疫荧光法检测汉坦病毒抗原，通过亚组计数和流式细胞术观察RGD对RTEC黏附的影响。本研究表明：（1）NE-ARF患者尿液中存在亚致死剂量的RTEC，可呈单层培养；（2）RTEC中存在NE抗原；（3）RGD可抑制RTEC的黏附。
