Yang L H, Zhang H Z, Zhang B, Chen F, Lai Z H, Xu L F, Jin X Q
Shenyang College of Pharmacy.
Yao Xue Xue Bao. 1992;27(9):679-83.
Four bufotoxins were isolated by means of preparative reversed phase HPLC from the water soluble fraction of the skin of Bufo bufo gargarizans Cantor. On the basis of their spectral data (UV, IR, 1HNMR, 13CNMR, EIMS and FDMS), amino acid analyses and chemical properties, they were identified as bufotalin 3-succinoylarginine ester(I), bufalin 3-succinoylarginine ester(II), cinobufagin 3-succinoylarginine ester(III), and resibufogenin 3-succinoylarginine ester(IV). Compound I is a new bufotoxin.
采用制备型反相高效液相色谱法从中华大蟾蜍皮肤的水溶性部分分离出四种蟾毒素。根据它们的光谱数据（紫外、红外、核磁共振氢谱、核磁共振碳谱、电子轰击质谱和场解吸质谱）、氨基酸分析和化学性质，将它们鉴定为蟾毒灵3 - 琥珀酰精氨酸酯（I）、蟾酥灵3 - 琥珀酰精氨酸酯（II）、华蟾酥毒基3 - 琥珀酰精氨酸酯（III）和脂蟾毒配基3 - 琥珀酰精氨酸酯（IV）。化合物I是一种新的蟾毒素。
