8,12-异前列腺素F2α-VI在大脑中的水平升高可将阿尔茨海默病与额颞叶痴呆区分开来。
Enhanced brain levels of 8,12-iso-iPF2alpha-VI differentiate AD from frontotemporal dementia.
作者信息
Yao Y, Zhukareva V, Sung S, Clark C M, Rokach J, Lee V M-Y, Trojanowski J Q, Praticò D
机构信息
Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia 19104, USA.
出版信息
Neurology. 2003 Aug 26;61(4):475-8. doi: 10.1212/01.wnl.0000070185.02546.5d.
OBJECTIVE
To quantify the isoprostane 8,12-iso-iPF2alpha-VI in brain tissues obtained from patients with AD, patients with frontotemporal dementia (FTD), and controls.
BACKGROUND
Enhanced brain oxidative stress with secondary damage to cellular macromolecules may play a role in the pathogenesis of AD and FTD. The isoprostane 8,12-iso-iPF2alpha-VI is a specific and sensitive marker of in vivo lipid peroxidation and is increased in AD.
METHODS
Levels of this isoprostane were determined by gas chromatography/negative ion chemical ionization mass spectrometry.
RESULTS
Levels of 8,12-iso-iPF2alpha-VI were markedly elevated in both frontal and temporal cortex of AD brains compared to the corresponding areas of FTD and controls. No significant difference in brain 8,12-iso-iPF2alpha-VI levels for any regions considered was observed between FTD and controls.
CONCLUSIONS
Lipid peroxidation is a feature of AD, but not FTD. The generation of 8,12-iso-iPF(2alpha)-VI in the brain is not a general or final common pathway of neurodegeneration, but may be relatively specific for disease processes in AD and not FTD.
目的
对取自阿尔茨海默病（AD）患者、额颞叶痴呆（FTD）患者及对照组的脑组织中的异前列腺素8,12-异-iPF2α-VI进行定量分析。
背景
脑部氧化应激增强并继发细胞大分子损伤可能在AD和FTD的发病机制中起作用。异前列腺素8,12-异-iPF2α-VI是体内脂质过氧化的一种特异且敏感的标志物，在AD中会升高。
方法
采用气相色谱/负离子化学电离质谱法测定该异前列腺素的水平。
结果
与FTD患者及对照组相应脑区相比，AD患者额叶和颞叶皮质中8,12-异-iPF2α-VI的水平显著升高。FTD患者与对照组之间，在所研究的任何脑区中，8,12-异-iPF2α-VI水平均未观察到显著差异。
结论
脂质过氧化是AD的一个特征，但不是FTD的特征。脑中8,12-异-iPF(2α)-VI的生成并非神经退行性变的普遍或最终共同途径，而可能相对特异地见于AD而非FTD的疾病进程。
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