Praticò D, MY Lee V, Trojanowski J Q, Rokach J, Fitzgerald G A
The Center for Experimental Therapeutics, Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine. Philadelphia, Pennsylvania, 19104, USA.
FASEB J. 1998 Dec;12(15):1777-83. doi: 10.1096/fasebj.12.15.1777.
Alzheimer's disease (AD) includes a group of dementing neurodegenerative disorders that have diverse etiologies but the same hallmark brain lesions. Since oxidative stress may play a role in the pathogenesis of AD and isoprostanes are chemically stable peroxidation products of arachidonic acid, we measured both iPF2alpha-III and iPF2alpha -VI using gas chromatography-mass spectrometry in AD and control brains. The levels of both isoprostanes, but not of 6-keto PGF1alpha, an index of prostaglandin production, were markedly elevated in both frontal and temporal poles of AD brains compared to the corresponding cerebella. Levels were also elevated compared to corresponding areas of brains from patients who had died with schizophrenia or Parkinson's disease or from nonneuropsychiatric disorders. iPF2alpha -IV, but not iPF2alpha-III, levels were higher in ventricular CSF of AD brains relative to the non-AD brains. These data suggest that specific isoprostane analysis may reflect increased oxidative stress in AD.
阿尔茨海默病（AD）包括一组具有不同病因但具有相同标志性脑损伤的痴呆性神经退行性疾病。由于氧化应激可能在AD的发病机制中起作用，而异前列腺素是花生四烯酸化学稳定的过氧化产物，我们使用气相色谱-质谱法在AD患者和对照者的大脑中测量了iPF2α-III和iPF2α-VI。与相应的小脑相比，AD大脑额叶和颞叶两极中两种异前列腺素的水平均显著升高，但作为前列腺素产生指标的6-酮-PGF1α水平未升高。与死于精神分裂症、帕金森病或非神经精神疾病患者大脑的相应区域相比，其水平也有所升高。相对于非AD大脑，AD大脑脑室脑脊液中iPF2α-IV水平升高，而iPF2α-III水平未升高。这些数据表明，特定异前列腺素分析可能反映了AD中氧化应激的增加。
