Tomasik A, Jacheć W, Skrzep-Poloczek B, Widera-Romuk E, Wodniecki J, Wojciechowska C
II Department of Cardiology, Silesian School of Medicine, Zabrze, Poland.
Scand J Clin Lab Invest. 2003;63(4):259-65.
There is growing experimental evidence to suggest the role of oxidatively modified low-density lipoprotein (LDL) in the initiation and progression of atherosclerosis. The oxidation of lipoprotein moiety causes modification of positively charged lysine residues and results in negative net charge of lipoprotein particles.
To measure the amount of circulating electronegatively charged LDL particles (LDL-) in plasma of patients with angiographically documented coronary artery disease (CAD).
Thirty patients were assigned to the study group (CAD+) and 10 patients to the control group (Ctrl). LDL- was quantitated in homogeneous LDL fractions obtained by ultracentrifugation, using ion exchange high performance liquid chromatography. Plasma lipids were measured using enzymatic kits.
The CAD+ group had significantly higher levels of LDL- in the whole LDL fraction (7.66+/-1.92 vs. 5.14+/-0.84%, p=0.0003). Moreover the CAD+ group had significantly higher levels of total cholesterol (255.4+/-35.1 vs. 210.4+/-22.4 mg/dL), LDL cholesterol (154.5+/-26.9 vs. 122.4+/-21.1 mg/dL) and significantly lower levels of high-density lipoprotein (HDL) cholesterol (40.4+/-9.4 vs. 51.0+/-11.5 mg/dL). LDL- remained significantly higher in the CAD+ group after adjustment for total cholesterol, LDL cholesterol and HDL cholesterol (6.3 vs. 5.14% at p=0.0095). There is a trend towards a positive correlation between LDL- levels and LDL cholesterol in the control group (Spearman R=0.55 at p=0.098).
Electronegatively charged LDL appears to be an additional hallmark of coronary artery disease, independently of established lipid risk factors. The trend towards a positive correlation between LDL cholesterol concentration and the level of LDL- in the control group may reflect the susceptibility of LDL cholesterol to autoxidation, Moreover, this may indicate other oxidative mechanisms in coronary artery disease. Nonetheless, further studies assessing the prognostic value of electronegatively charged LDLs are necessary.
越来越多的实验证据表明氧化修饰的低密度脂蛋白（LDL）在动脉粥样硬化的发生和发展中起作用。脂蛋白部分的氧化导致带正电荷的赖氨酸残基发生修饰，并使脂蛋白颗粒带负净电荷。
测量经血管造影证实患有冠状动脉疾病（CAD）的患者血浆中带负电荷的LDL颗粒（LDL-）的含量。
30例患者被分配到研究组（CAD+），10例患者被分配到对照组（Ctrl）。使用离子交换高效液相色谱法对通过超速离心获得的均一LDL组分中的LDL-进行定量。使用酶试剂盒测量血浆脂质。
CAD+组整个LDL组分中的LDL-水平显著更高（7.66±1.92%对5.14±0.84%，p=0.0003）。此外，CAD+组的总胆固醇水平显著更高（255.4±35.1对210.4±22.4mg/dL），LDL胆固醇水平显著更高（154.5±26.9对122.4±21.1mg/dL），而高密度脂蛋白（HDL）胆固醇水平显著更低（40.4±9.4对51.0±11.5mg/dL）。在调整总胆固醇、LDL胆固醇和HDL胆固醇后，CAD+组的LDL-水平仍显著更高（6.3对5.14%，p=0.0095）。对照组中LDL-水平与LDL胆固醇之间存在正相关趋势（Spearman相关系数R=0.55，p=0.098）。
带负电荷的LDL似乎是冠状动脉疾病的另一个标志，独立于已确定的脂质危险因素。对照组中LDL胆固醇浓度与LDL-水平之间的正相关趋势可能反映了LDL胆固醇对自氧化的敏感性。此外，这可能表明冠状动脉疾病中的其他氧化机制。尽管如此，有必要进一步研究评估带负电荷的LDL的预后价值。
