Sabat S C, Mohanty P
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Indian J Biochem Biophys. 1992 Dec;29(6):490-3.
Electron transport activity of beet spinach thylakoids was enhanced in the presence of aliphatic amine, DABCO (1,4-diazobicyclo (2,2,2) octane), a hydrophilic proton trapping agent. The extent of stimulation was pH-dependent and similar to the effect of the uncoupler ammonium chloride on electron transport. The stimulation of whole-chain (H2O-->MV) electron transport activity was observed only at high (rate-saturating) light intensity. The light-induced proton uptake coupled to electron transport of thylakoid was also arrested by DABCO, suggesting that DABCO uncouples thylakoid phosphorylation by proton trapping.
在亲水性质子捕获剂脂肪族胺1,4 - 二氮杂双环[2.2.2]辛烷（DABCO）存在的情况下，甜菜菠菜类囊体的电子传递活性增强。刺激程度取决于pH值，并且与解偶联剂氯化铵对电子传递的影响相似。仅在高光强（速率饱和）下观察到对全链（H₂O→甲基紫精）电子传递活性的刺激。DABCO也抑制了类囊体电子传递所伴随的光诱导质子摄取，这表明DABCO通过捕获质子使类囊体磷酸化解偶联。
