Lashchevskiĭ V V, Kovalenko N K
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Mikrobiol Z. 2003 May-Jun;65(3):46-53.
Primers LP1-LP2 to the gene 16S rRNA have been developed, which permit to differentiate lactic acid bacteria: Lactobacillus plantarum, L. delbrueckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus. The strain-specific and species-specific differentiations are possible under different annealing temperature. Additional fragments, which are synthesized outside the framework of gene 16S rRNA reading, provide for the strain-specific type of differentiation, and the fragment F864 read in the gene 16S rRNA permits identifying L. plantarum.
已开发出针对16S rRNA基因的引物LP1-LP2,可用于区分乳酸菌:植物乳杆菌、德氏乳杆菌保加利亚亚种和唾液链球菌嗜热亚种。在不同的退火温度下,可以实现菌株特异性和物种特异性的区分。在16S rRNA阅读框架之外合成的额外片段可实现菌株特异性的区分类型,而在16S rRNA基因中读取的片段F864可用于鉴定植物乳杆菌。