Bulychev L E, Poryvaev V D, Ryzhikov A B, Karpyshev N N, Alekseeva A G, Goncharova E P, Pliasunov I V
Vopr Virusol. 2003 Jul-Aug;48(4):45-7.
Combined application of ridostine with catonic liposomes was shown to essentially enhance the interferon-inducing and antiviral activity of the former in experiments with cell cultures L-929, which is apparently related with an improved efficiency of intracellular delivery of dsRNA. A comparative study demonstrated that ridostine, when combined with liposomes, is needed by 10(3)-10(4) times less as when it is used alone. A pretreatment of the cellular monolayer by cationic liposomes contributes also to enhancing the activity of ridostine, which can be explained by an enhanced permeability of cells for dsRNA holding on-for as long as 30 minutes after the removal of liposomes from the liquid culture. A separate successive administration of, first, liposomes and, then, of ridostine in BALB/c mice (20 mg/kg) leads to a more intensified induction of interferon in the upper respiratory tract tissues as compared with the administration of ridostine alone.
在L-929细胞培养实验中，已表明利多司汀与阳离子脂质体联合应用能显著增强前者的干扰素诱导和抗病毒活性，这显然与双链RNA细胞内递送效率的提高有关。一项比较研究表明，与单独使用时相比，利多司汀与脂质体联合使用时的用量要少10³至10⁴倍。阳离子脂质体对细胞单层进行预处理也有助于增强利多司汀的活性，这可以解释为细胞对双链RNA的通透性增强，在从液体培养物中去除脂质体后，这种增强的通透性可持续长达30分钟。在BALB/c小鼠（20mg/kg）中，先单独给予脂质体，然后再给予利多司汀，与单独给予利多司汀相比，会导致上呼吸道组织中干扰素的诱导作用更强。
