Timofeev I V, Chaplygina S R, Zorin V V, Khromykh A A, Kolokol'tsov A A
Antibiot Med Biotekhnol. 1987 Feb;32(2):144-7.
Interferon-inducing and antiviral effects of natural dsRNA preparations of phage phi 6 and yeast cells were studied in the culture of murine cells L-929 and on random bred albino mice. Both the preparations showed interferon inducing activity in the cell culture. However, for realization of their effect modification of the surface cell membrane by polycation exchange resin (DEAE-dextran) was required. The interferon-inducing activity of both of the natural dsRNA in the mice was high. The maximum interferon titers (1280-5120 units/ml) in blood serum were observed 4-6 hours after the inductor intraperitoneal administration. The interferon-inducing activity of the phage dsRNA was high in the cell culture and yeast dsRNA--in mice, respectively. Both the inductors had antiviral activity and protected 15 to 38.9 per cent of the experimental animals from the effect of 100 LD50 of the murine encephalomyocarditis virus and 10 LD50 of the influenza virus A/Aichi 2/68 (H3N2).
在小鼠L-929细胞培养物和随机繁殖的白化小鼠中研究了噬菌体φ6和酵母细胞的天然双链RNA制剂的干扰素诱导和抗病毒作用。两种制剂在细胞培养中均显示出干扰素诱导活性。然而，为了实现其作用，需要用聚阳离子交换树脂（DEAE-葡聚糖）对细胞表面膜进行修饰。两种天然双链RNA在小鼠中的干扰素诱导活性都很高。在诱导剂腹腔注射后4-6小时，血清中观察到最大干扰素滴度（1280-5120单位/毫升）。噬菌体双链RNA在细胞培养中的干扰素诱导活性高，酵母双链RNA在小鼠中的干扰素诱导活性高。两种诱导剂均具有抗病毒活性，并保护15%至38.9%的实验动物免受100个半数致死剂量的小鼠脑心肌炎病毒和10个半数致死剂量的甲型流感病毒A/爱知2/68（H3N2）的影响。
