以铽离子作为荧光探针测定尿液中的环丙沙星

[Determination of ciprofloxacin in urine using terbium ion as fluorescent probe].

作者信息

Wang L, Sang L H, Wu Y L, Cheng X M, Su Z D, Yu A H

机构信息

Department of Pharmacy, Shandong Medical University, Jinan 250012, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Oct;21(5):691-3.

PMID:12945334

Abstract

Factors effecting the fluorescence characteristics of the reaction CPFX-Tb were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Tb. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 x 10(-8)-1.0 x 10(-6) mol.L-1. The regression equation is F = 1.57 + 4.87 x 10(8) c, r = 0.9999 with the detection limit of 5.0 x 10(-9) mol.L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe.

摘要

深入研究了影响反应CPFX-Tb荧光特性的因素。确定了实验条件对荧光强度的影响。在pH 6.0介质中，CPFX-Tb络合物荧光体系可发射Tb的本征荧光。激发波长和发射波长分别为328 nm和545 nm。线性范围为1.0×10⁻⁸ - 1.0×10⁻⁶ mol·L⁻¹。回归方程为F = 1.57 + 4.87×10⁸c，r = 0.9999，检测限为5.0×10⁻⁹ mol·L⁻¹。以铽离子为荧光探针测定尿液中痕量环丙沙星，结果令人满意。