高效液相色谱法同时测定大黄及实验动物体内的羟基蒽醌类成分

Simultaneous determination of hydroxyanthraquinones in rhubarb and experimental animal bodies by high-performance liquid chromatography.

作者信息

Ding Mingyu, Ma Shuaiwu, Liu Delin

机构信息

Department of Chemistry, Tsinghua University, Beijing 100084, China.

出版信息

Anal Sci. 2003 Aug;19(8):1163-5. doi: 10.2116/analsci.19.1163.

DOI:10.2116/analsci.19.1163

PMID:12945670

Abstract

A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of five hydroxyanthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion) in Rhubarb and experimental animal bodies. A Zorbax SB-C18 column (250 mm x 4.6 mm i.d., 5 microm) and a methanol-0.5% acetic acid (85:15, v/v) mobile phase were used for the separation. The detection wavelength of a diode array detector (DAD) was set at 254 nm. Regression equations revealed a linear relationship (R2>0.9996) between the mass of hydroxyanthraquinones injected and the peak areas detected by DAD. The detection limits (S/N=3) ranged from 0.35 ng to 3.13 ng, and the recoveries ranged from 83% to 103% for different hydroxyanthraquinones. This method is simple, sensitive and suitable for the analysis of hydroxyanthraquinones in medicinal materials and pharmacological experiment samples.

摘要

建立了一种简单可靠的高效液相色谱（HPLC）法，用于同时测定大黄及实验动物体内的5种羟基蒽醌（芦荟大黄素、大黄酸、大黄素、 Chrysophanol和大黄酚）。采用Zorbax SB-C18柱（250 mm×4.6 mm内径，5 μm）和甲醇-0.5%乙酸（85:15，v/v）流动相进行分离。二极管阵列检测器（DAD）的检测波长设定为254 nm。回归方程显示，注入的羟基蒽醌质量与DAD检测到的峰面积之间存在线性关系（R2>0.9996）。不同羟基蒽醌的检测限（S/N=3）为0.35 ng至3.13 ng，回收率为83%至103%。该方法简便、灵敏，适用于药材和药理实验样品中羟基蒽醌的分析。