Zhang Hong-Quan, Zhou Cheng-Hua, Wu Yu-Qing
Medical and Pharmacological Institute, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
World J Gastroenterol. 2005 May 28;11(20):3147-50. doi: 10.3748/wjg.v11.i20.3147.
To investigate the effect of emodin on small intestinal peristalsis of mice and to explore its relevant mechanisms.
The effect of emodin on small intestinal peristalsis of mice was observed by charcoal powder propelling test of small intestine. The contents of motilin and somatostatin in small intestine of mice were determined by radioimmunoassay. The electrical potential difference (PD) related to Na+ and glucose transport was measured across the wall of reverted intestinal sacs. Na+-K+-ATPase activity of small intestinal mucosa was measured by spectroscopic analysis.
Different dosages of emodin can improve small intestinal peristalsis of mice. Emodin increased the content of motilin, while reduced the content of somatostatin in small intestine of mice significantly. Emodin 0.2, 0.4, 0.8, and 1.6 g/L decreased PD when there was glucose. However, emodin had little effect when glucose was free. The Na+-K+-ATPase activity of small intestinal mucosa of mice in emodin groups was inhibited obviously.
Emodin can enhance the function of small intestinal peristalsis of mice by mechanisms of promoting secretion of motilin, lowering the content of somatostatin and inhibiting Na+-K+-ATPase activity of small intestinal mucosa.
研究大黄素对小鼠小肠蠕动的影响并探讨其相关机制。
通过小肠炭末推进试验观察大黄素对小鼠小肠蠕动的影响。采用放射免疫分析法测定小鼠小肠中胃动素和生长抑素的含量。跨翻转肠囊壁测量与钠和葡萄糖转运相关的电位差（PD）。用分光光度法测定小肠黏膜的钠钾ATP酶活性。
不同剂量的大黄素均可改善小鼠小肠蠕动。大黄素显著增加小鼠小肠中胃动素的含量，同时降低生长抑素的含量。当有葡萄糖存在时，0.2、0.4、0.8和1.6 g/L的大黄素可降低PD。然而，当无葡萄糖时，大黄素影响很小。大黄素组小鼠小肠黏膜的钠钾ATP酶活性明显受到抑制。
大黄素可通过促进胃动素分泌、降低生长抑素含量及抑制小肠黏膜钠钾ATP酶活性来增强小鼠小肠蠕动功能。
