Institute of Integrated Traditional Chinese and Western Medicine, and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
Chin J Integr Med. 2012 Sep;18(9):690-8. doi: 10.1007/s11655-012-1053-7. Epub 2012 Aug 31.
To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment.
Anthraquinones in water extract of rhubarb (WER), in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER.
Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly.
Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
检测具有颅脑保护作用的泻药水提取物（WER）的吸收生物活性化合物，以进行质量控制评估。
采用本研究建立的超高效液相色谱-光电二极管阵列检测法（UPLC-PDA）分别检测 WER 水提物、颅脑损伤（TBI）患者脑脊液和 TBI 大鼠损伤侧皮质中的蒽醌类化合物。检测吸收到损伤皮质中的蒽醌类化合物对 TBI 大鼠超氧化物歧化酶（SOD）活性的影响。评估吸收到靶器官的抗氧化性蒽醌类化合物，以控制 WER 的质量。
WER 中的蒽醌类化合物为芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚。在这种色谱条件下，仅最后一种蒽醌类化合物在 CSF 和损伤侧皮质中被发现。大黄素甲醚能显著增加 TBI 大鼠的 SOD 活性。
大黄素甲醚通过抗氧化途径成为治疗颅脑损伤的泻下药水提取物的主要活性化合物。根据我们的策略，大黄素甲醚的探索为治疗 TBI 损伤的 WER 提供了一种新的质量控制方法的可能性。
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