Fung D T C, Ng G Y F, Leung M C P, Tay D K C
Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Kowloon, Hong Kong.
Connect Tissue Res. 2003;44(1):2-11.
This study compared the collagen fibril diameter distribution among six anatomical sites of the rat medial collateral ligament (MCL). Ultrathin MCL sections from 4 male Sprague-Dawley rats were examined electron microscopically. With an automated quantitation method, 41,638 fibrils were measured and compared among the periphery and core regions of the femoral, middle, and tibial portions of the MCL. Results demonstrated significant difference (p < .0033) in mean fibril diameter distribution among the six sites. The mass-averaged diameters of the core and peripheral fibrils were between 175.53 to 190.82 nm and 88.47 to 109.18 nm, respectively, with the peripheral fibrils more homogeneous in size. The fibrils occupied 36.7% to 57.1% of the cross-sectional area of the ligament. About 50% of the fibrils had an oblique factor of 0.8-1.0, implying that most fibrils were aligned longitudinally. This study has provided a detailed profile of the collagen fibril distributions in rat MCL.
本研究比较了大鼠内侧副韧带（MCL）六个解剖部位的胶原纤维直径分布。对4只雄性Sprague-Dawley大鼠的MCL超薄切片进行了电子显微镜检查。采用自动定量方法，测量并比较了MCL股骨部、中部和胫骨部外周和核心区域的41638根纤维。结果表明，六个部位的平均纤维直径分布存在显著差异（p < 0.0033）。核心纤维和外周纤维的质量平均直径分别在175.53至190.82纳米和88.47至109.18纳米之间，外周纤维的尺寸更均匀。纤维占韧带横截面积的36.7%至57.1%。约50%的纤维倾斜因子为0.8-1.0，这意味着大多数纤维是纵向排列的。本研究提供了大鼠MCL中胶原纤维分布的详细概况。
