Binkley J M, Peat M
Clin Orthop Relat Res. 1986 Feb(203):301-8.
Rat medial collateral ligaments from one group of animals were tensile tested following immobilization. Ligaments from a second group of animals were examined using transmission electron microscopy to determine collagen fibril cross-sectional area and density. Immobilized and contralateral control ligaments were compared to determine the effect of immobilization on these parameters. There was a significant decrease in the linear stress, maximum stress, and stiffness of immobilized ligaments. There was no significant difference in the strain values reported. There was a significant decrease in the proportion of smaller, cross-sectional area fibrils (0-10,000 nm2) following immobilization. There was also a significant increase in the proportion of larger (30,000-40,000 nm2 and 40,000-50,000 nm2) fibrils following immobilization. This finding was attributed to a decreased synthesis and decreased degradation of collagen during immobilization; post-immobilization, the density of collagen fibrils in the ligament decreased. There may be a relationship between ultrastructural and mechanical alterations following immobilization. Significant clinical implications are apparent in the altered mechanical properties following immobilization.
对一组动物的大鼠内侧副韧带进行固定后拉伸测试。对另一组动物的韧带进行透射电子显微镜检查，以确定胶原纤维的横截面积和密度。比较固定侧和对侧对照韧带，以确定固定对这些参数的影响。固定韧带的线性应力、最大应力和刚度显著降低。报告的应变值无显著差异。固定后，横截面积较小的纤维（0 - 10,000平方纳米）比例显著降低。固定后，较大纤维（30,000 - 40,000平方纳米和40,000 - 50,000平方纳米）的比例也显著增加。这一发现归因于固定期间胶原蛋白合成减少和降解减少；固定后，韧带中胶原纤维的密度降低。固定后超微结构和力学改变之间可能存在关联。固定后力学性能改变具有明显的临床意义。
