Woodward Mark, Lowe Gordon, Rumley Ann, Imhof Armin, Koenig Wolfgang
Institute for International Health, University of Sydney, Australia.
Blood Coagul Fibrinolysis. 2003 Jun;14(4):417-20. doi: 10.1097/00001721-200306000-00015.
To establish whether plasma viscosity could be accurately measured from frozen-stored (instead of fresh) plasma samples in epidemiological studies, we prospectively compared fresh and frozen-stored samples from 1361 men and women aged 25-74 years participating in the Glasgow MONICA survey. Mean plasma viscosity (37 degrees C) was significantly higher (P < 0.0001) in fresh samples (mean = 1.310 mPa/s, standard deviation = 0.079 mPa/s) compared with frozen samples (mean = 1.278 mPa/s, standard deviation = 0.075 mPa/s). However, this difference (mean = 0.030 mPa/s, 95% confidence interval = 0.029-0.031) did not vary significantly with increasing viscosity (kappa statistic for consistency of categorization by thirds = 0.824, standard error = 0.021). We conclude that frozen samples are acceptable for epidemiological studies of plasma viscosity, which should increase their utilization.
为了确定在流行病学研究中是否可以从冷冻保存（而非新鲜）的血浆样本中准确测量血浆粘度，我们前瞻性地比较了参与格拉斯哥莫尼卡调查的1361名年龄在25 - 74岁的男性和女性的新鲜血浆样本和冷冻保存血浆样本。与冷冻样本（均值 = 1.278 mPa/s，标准差 = 0.075 mPa/s）相比，新鲜样本（均值 = 1.310 mPa/s，标准差 = 0.079 mPa/s）的平均血浆粘度（37摄氏度）显著更高（P < 0.0001）。然而，这种差异（均值 = 0.030 mPa/s，95%置信区间 = 0.029 - 0.031）并未随着粘度增加而显著变化（按三分法分类一致性的kappa统计量 = 0.824，标准误 = 0.021）。我们得出结论，冷冻样本可用于血浆粘度的流行病学研究，这应会提高其利用率。
