Hua Zhaozhe, Chen Jian, Lun Shiyi, Wang Xiaorong
School of Biotechnology, Southern Yangtze University (Wuxi University of Light Industry), 214036 Wuxi, China.
Water Res. 2003 Oct;37(17):4143-50. doi: 10.1016/S0043-1354(03)00380-4.
The objective of this study was to investigate the influence of the biosurfactant BS-UC on surface properties of microbial cells and biodegradation of petroleum hydrocarbons. The biosurfactant BS-UC was produced by Candida antarctica from n-undecane as the substrate. It was found that the addition of BS-UC influenced positively the emulsification and the biodegradation of a variety of n-alkanes substrates. Besides, BS-UC also changed the hydrophobicity and zeta potential of the cell surface, and thus made the microbial cell attach to the hydrophobic substrate easily. The cell transport experiment also indicated that the addition of BS-UC changed both zeta potential of the cell and that of the porous media, and improved the retention of the cells in the media. So it will make BS-UC a promising choice for use in bioremediation of petroleum contamination.
本研究的目的是调查生物表面活性剂BS-UC对微生物细胞表面性质以及石油烃生物降解的影响。生物表面活性剂BS-UC由南极假丝酵母以正十一烷为底物产生。研究发现,添加BS-UC对多种正构烷烃底物的乳化和生物降解有积极影响。此外,BS-UC还改变了细胞表面的疏水性和zeta电位,从而使微生物细胞易于附着在疏水底物上。细胞传输实验还表明,添加BS-UC改变了细胞和多孔介质的zeta电位,并提高了细胞在介质中的保留率。因此,这将使BS-UC成为石油污染生物修复的一个有前景的选择。