Chen Gang, Qiu Xiaoqing, Lu Xiaofeng, Pei Yan, Wei Qiou, Cheng Jingqiu
Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):220-2.
To investigate the inhibition property of a novel antifungal polypeptide APS in vitro and its possible antifungal mechanism.
The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of APS against different fungi were detected by microdilution test. And the voltaic change of planar lipid bilayer was observed during APS treatment.
APS at low MIC and MBC was shown to have effects on phytopathogenic fungi tested except aspergillus fumigatus, the minimum values of MIC and MBC being 1.43 micrograms/ml and 1.78 micrograms/ml respectively. After APS was put in, the transmembrane current could be recorded.
APS has strong inhibitory effects on the germination of spores of several phytopathogenic fungi. Its antifungal mechanism is likely to form persistent pores on the cell membrane, thus causing membrane permeation and the death of fungi.
研究新型抗真菌多肽APS的体外抑菌特性及其可能的抗真菌机制。
采用微量稀释法检测APS对不同真菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。并观察APS处理过程中平面脂质双分子层的电压变化。
低MIC和MBC的APS对除烟曲霉外的受试植物病原真菌有作用，MIC和MBC的最小值分别为1.43微克/毫升和1.78微克/毫升。加入APS后，可以记录到跨膜电流。
APS对几种植物病原真菌的孢子萌发有较强的抑制作用。其抗真菌机制可能是在细胞膜上形成持久的孔道，从而导致膜通透性增加和真菌死亡。
