[利用双向凝胶电泳分析面神经再生微环境及其对针刺的反应]
[Using two-dimensional gel electrophoresis to analyze the regeneration microenvironment of facial nerve and its response to acupuncture].
作者信息
Yang Hui, Yang Cairong, Lu Xiaofeng, Liang Chuanyu, Gao Bingqing
机构信息
Otonasolaryngeal Department, West China Hospital, Sichuan Universty, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):242-4.
OBJECTIVE
To investigate the distribution of proteins in the regeneration microenvironment of facial nerve and its response to acupuncture.
METHODS
We created an animal model using rabbit to imitate the regeneration microenvironment of facial nerve and analyzed the proteins in the microenvironment through the two-dimensional gel electrophoresis.
RESULTS
We found that most proteins in the regeneration fluid were under relative molecular mass 80 x 10(3) and in the range of pI 4-7. An average of 800 +/- 53 protein spots was found in the control group compared to the 850 +/- 78 spots in the acupuncture group. In the matching spots, more than ten proteins were different between the two groups.
CONCLUSION
There is no evidence to show that acupuncture has a positive effect on the regeneration microenvironment of facial nerve.
目的
探讨面神经再生微环境中蛋白质的分布及其对针刺的反应。
方法
采用家兔建立动物模型以模拟面神经再生微环境，并通过二维凝胶电泳分析微环境中的蛋白质。
结果
我们发现再生液中的大多数蛋白质相对分子质量低于80×10³，等电点范围为4-7。对照组平均发现800±53个蛋白点，针刺组为850±78个蛋白点。在匹配的蛋白点中，两组之间有十多种蛋白质存在差异。
结论
没有证据表明针刺对面神经再生微环境有积极作用。
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