Vilela Daniela Salgado Alves, Lazarini Paulo Roberto, Da Silva Ciro Ferreira
Otorhinolaryngology Department, Santa Casa São Paulo, Faculty of Medical Sciences, São Paulo, Brazil.
Acta Otolaryngol. 2008 Sep;128(9):1048-52. doi: 10.1080/00016480701827525.
Hyperbaric oxygen treatment (HBOT) promoted an increase of the mean axonal diameter in the group evaluated 2 weeks after lesion induction, which suggests a more advanced regeneration process. However, the number of myelin nerve fibers of the facial nerve of the rabbits was similar when compared to the control and treatment groups, in both evaluation periods.
To evaluate the effect of HBOT on the histological pattern of the facial nerve in rabbits exposed to a nerve crush injury.
Twenty rabbits were exposed to facial nerve crush injury. Ten rabbits received HBOT, 10 rabbits comprised the control group. The rabbits were sacrificed 2 and 4 weeks after the trauma. Qualitative morphological analysis, measurement of the external axonal diameters and myelin fiber count were carried out in an area of 185 000 microm2.
There was an increase in the area of the axons and thicker myelin in the 2 weeks treatment group in comparison with the control group. The mean diameter of the axons was of 2.34 microm in the control group and of 2.81 microm in the HBOT group, with statistically significant differences. The 2 week control group had a mean number of myelin fibers of 1865.2 +/- 664, and the HBOT group had a mean number of 2026.3 +/- 302; this was not statistically significant. The 4 week control group presented a mean of 2495.1 +/- 479 fibers and the HBOT group presented a mean of 2359.9 +/- 473; this was not statistically significant.
高压氧治疗（HBOT）促使损伤诱导后2周评估的组中轴突平均直径增加，这表明再生过程更高级。然而，在两个评估期，与对照组和治疗组相比，兔子面神经的髓鞘神经纤维数量相似。
评估高压氧治疗对遭受神经挤压伤的兔子面神经组织学模式的影响。
20只兔子遭受面神经挤压伤。10只兔子接受高压氧治疗，10只兔子组成对照组。在创伤后2周和4周处死兔子。在185000平方微米的区域进行定性形态学分析、轴突外径测量和髓鞘纤维计数。
与对照组相比，2周治疗组的轴突面积增加且髓鞘更厚。对照组轴突的平均直径为2.34微米，高压氧治疗组为2.81微米，差异有统计学意义。2周对照组的髓鞘纤维平均数为1865.2±664，高压氧治疗组为2026.3±302；差异无统计学意义。4周对照组的纤维平均数为2495.1±479，高压氧治疗组为2359.9±473；差异无统计学意义。
