[毛细管电泳法测定谷物中的玉米赤霉烯酮及其代谢产物]
[Determination of zearalenone and its metabolites in grains by capillary electrophoresis].
作者信息
Zeng Hongyan, Li Yuanqian, Jin Jun, Sun Huiqin
机构信息
Department of Sanitary Technology, West China School of Pulic Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Apr;34(2):333-6.
OBJECTIVE
To establish a method for the determination of zearalenone and its metabolites in grains by capillary electrophoresis.
METHODS
The samples were extracted with liquid-liquid partition and ultrasonic extraction, cleaned up with C18 cartridges, then determined by micellar electrokinetic capillary chromatography (MEKC).
RESULTS
The limits of detection for Zearalenone (ZON), alpha-Zearalenol (alpha-ZOL), beta-Zearalenol (beta-ZOL), Aflatoxin B1 (AFT B1) and Ochratoxin A (Och A) were 0.0084, 0.081, 0.14, 0.0016 and 0.031 microgram/ml, respectively. Recoveries of all compounds were in the range of 77.9%-103.1%. Relative standard deviations (RSDs) were 0.63%-1.98%.
CONCLUSION
This sensitive and accurate method can be used in the determination of zearalenone and its metabolites in grains.
目的
建立一种用毛细管电泳法测定谷物中玉米赤霉烯酮及其代谢物的方法。
方法
样品采用液-液分配和超声提取法提取，用C18柱净化，然后用胶束电动毛细管色谱法（MEKC）测定。
结果
玉米赤霉烯酮（ZON）、α-玉米赤霉醇（α-ZOL）、β-玉米赤霉醇（β-ZOL）、黄曲霉毒素B1（AFT B1）和赭曲霉毒素A（Och A）的检测限分别为0.0084、0.081、0.14、0.0016和0.031微克/毫升。所有化合物的回收率在77.9%-103.1%之间。相对标准偏差（RSD）为0.63%-1.98%。
结论
该灵敏准确的方法可用于测定谷物中玉米赤霉烯酮及其代谢物。
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