Feige K, Ehrat F B, Kästner S B R, Schwarzwald C C
Equine Clinic, Faculty of Veterinary Medicine, University of Zurich, Zurich, Switzerland.
J Vet Med A Physiol Pathol Clin Med. 2003 May;50(4):185-9. doi: 10.1046/j.1439-0442.2003.00528.x.
The purpose of this study was to evaluate plasmapheresis as a method for plasma extraction in comparison with centrifugation or gravity sedimentation. The study was designed as a cross over trial with six Freiberger horses undergoing plasma donation by plasmapheresis followed by whole-blood donation and subsequent plasma production 4 weeks later. Automated plasmapheresis and whole-blood donation were well tolerated in all horses. The plasmapheresis method achieved an almost complete removal of erythrocytes and leucocytes from plasma at all flow rates. After blood bag centrifugation, significantly more erythrocytes (P < 0.01) and leucocytes (P < 0.01) were present in the prepared plasma compared with plasmapheresis plasma. Plasma prepared by gravity sedimentation contained significantly more erythrocytes and leucocytes than plasma prepared with the other techniques (P < 0.01), and platelet aggregation was observed. The coagulation proteins and the total protein content of plasma prepared after plasmapheresis and blood bag centrifugation did not differ significantly from values measured prior to collection (P > 0.05). However, the activity of factor VIII was significantly lower 24 h after gravity sedimentation of blood than activity prior to blood collection (P < 0.01). In conclusion, automated plasmapheresis is the method of choice for the production of high quality equine plasma.
本研究的目的是评估血浆置换作为一种血浆提取方法，并与离心法或重力沉降法进行比较。该研究设计为交叉试验，六匹弗赖贝格马先通过血浆置换进行血浆捐献，然后进行全血捐献，并在4周后进行后续血浆制备。所有马匹对自动血浆置换和全血捐献的耐受性良好。在所有流速下，血浆置换法几乎能完全从血浆中去除红细胞和白细胞。与血浆置换血浆相比，血袋离心后制备的血浆中红细胞（P < 0.01）和白细胞（P < 0.01）明显更多。重力沉降法制备的血浆中红细胞和白细胞比其他技术制备的血浆明显更多（P < 0.01），并且观察到血小板聚集。血浆置换和血袋离心后制备的血浆中凝血蛋白和总蛋白含量与采集前测量的值相比无显著差异（P > 0.05）。然而，血液重力沉降24小时后因子VIII的活性明显低于采血前的活性（P < 0.01）。总之，自动血浆置换是生产高质量马血浆的首选方法。
