Nadin-Davis S A, Simani S, Armstrong J, Fayaz A, Wandeler A I
Centre of Expertise for Rabies, Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, 3851 Fallowfield Road, Nepean, Ontario, Canada K2H 8P9.
Epidemiol Infect. 2003 Aug;131(1):777-90. doi: 10.1017/s095026880300863x.
A molecular epidemiological study of 48 recent rabies isolates recovered from cases reported throughout Iran identified three distinct viral variants, the evolutionary origins of which were identified by phylogenetic comparison with rabies viruses originating from Europe and Asia. Members of group 1 (15 isolates) were recovered from the northern half of the country only, while those of group 2 (31 isolates) were widely dispersed; both groups clustered within the widely disseminated cosmopolitan lineage. The two isolates of group 3 were detected in the northeastern tip of the country only and belonged to the Arctic strain. Rapid variant discrimination tools, employing restriction fragment length polymorphisms applied to amplified fragments of the viral genome, were devised whilst antigenic characterization of representative viruses identified a small panel of monoclonal antibodies that were also discriminatory. The future application of such methods should provide valuable epidemiological information on rabies incidence in Iran.
一项对从伊朗各地报告的病例中分离出的48株近期狂犬病病毒进行的分子流行病学研究，鉴定出三种不同的病毒变体，通过与源自欧洲和亚洲的狂犬病病毒进行系统发育比较确定了它们的进化起源。第1组（15株分离株）仅从该国北半部分离得到，而第2组（31株分离株）分布广泛；两组均聚集在广泛传播的世界性谱系内。第3组的两株分离株仅在该国最东北部被检测到，属于北极毒株。利用限制性片段长度多态性对病毒基因组扩增片段进行分析，设计了快速变体鉴别工具，同时对代表性病毒的抗原特性进行鉴定，确定了一小部分具有鉴别能力的单克隆抗体。这些方法的未来应用应为伊朗狂犬病发病率提供有价值的流行病学信息。
