Bernardi F, Nadin-Davis S A, Wandeler A I, Armstrong J, Gomes A A B, Lima F S, Nogueira F R B, Ito F H
Department of Preventive Veterinary Medicine and Animal Health, FMVZ-USP, Av. Professor Dr Orlando Marques de Paiva 87, 05508-000 Cidade Universitária, São Paulo-SP, Brazil.
Rabies Centre of Expertise, Canadian Food Inspection Agency, Ottawa Laboratory-Fallowfield, 3851 Fallowfield Road, Ottawa, Canada K2H 8P9.
J Gen Virol. 2005 Nov;86(Pt 11):3153-3162. doi: 10.1099/vir.0.81223-0.
Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.
从巴西多个地区的多种动物物种中收集了50株狂犬病病毒，通过对P基因中央可变区进行部分测序对其进行了特征分析，该区域是敏感分子流行病学研究的一个有用位点。对这些序列进行系统发育分析，包括与从美洲各地分离出的其他狂犬病病毒株进行比较，确定了巴西病毒的三个主要组，分别命名为BRL - 1至BRL - 3。在陆生食肉动物中发现了BRL - 1，它与世界范围内谱系的其他美洲毒株聚类。BRL - 2包括从两种非吸血蝙蝠中分离出的两个不同毒株，它们与北美食虫蝙蝠衍生的毒株有进化联系。BRL - 3由来自吸血蝙蝠和可能通过与吸血蝙蝠接触而感染的家畜物种的分离株组成。陆生组进一步细分为三个亚型：BRL - 1a仅与狗和猫有关，而BRL - 1b和BRL - 1c仅在苍狐中发现。这些观察结果有力地支持了巴西苍狐作为狂犬病宿主的作用。用一组抗狂犬病单克隆抗体（mAb）对代表性巴西狂犬病病毒进行筛选，确定了一小部分可用于区分本研究中通过基因分类定义的所有巴西亚组的单克隆抗体。
