Walser Jean-Claude, Sperisen Christoph, Soliva Marco, Scheidegger Christoph
WSL Swiss Federal Research Institute, Zürcherstrasse 111, CH-8903 Birmensdorf, Switzerland.
Fungal Genet Biol. 2003 Oct;40(1):72-82. doi: 10.1016/s1087-1845(03)00080-x.
We isolated 12 microsatellite loci for the epiphytic lichen-forming ascomycete Lobaria pulmonaria and studied their patterns of variation within and among populations from Canada and Switzerland. Even though several microsatellites exhibited high levels of variability at different spatial scales, we did not find any evidence for intrathalline variation. Most of the genetic variation was attributed to differences among individuals within populations. High genetic variation was also detected among L. pulmonaria samples taken from individual trees, suggesting that either multiple colonization events had occurred or that local recombination is frequent. The geographically structured distribution of alleles from several microsatellites indicated that L. pulmonaria from Canada and Switzerland represent two distinct evolutionary lineages. The potential to identify multiple alleles, and their transferability to closely related species, make microsatellites an ideal tool to study dispersal, population differentiation, and microevolution in lichens.
我们分离出了12个微卫星位点用于研究附生地衣形成子囊菌肺衣，并研究了它们在加拿大和瑞士种群内部和种群之间的变异模式。尽管几个微卫星在不同空间尺度上表现出高度变异性，但我们没有发现任何菌体内变异的证据。大部分遗传变异归因于种群内个体间的差异。从单株树上采集的肺衣样本之间也检测到了高遗传变异，这表明要么发生了多次定殖事件，要么局部重组频繁。几个微卫星等位基因的地理结构分布表明，来自加拿大和瑞士的肺衣代表两个不同的进化谱系。识别多个等位基因的潜力及其向近缘物种的转移性，使微卫星成为研究地衣扩散、种群分化和微进化的理想工具。
