枯草芽孢杆菌的快速表面运动依赖于细胞外表面活性素和钾离子。
Rapid surface motility in Bacillus subtilis is dependent on extracellular surfactin and potassium ion.
作者信息
Kinsinger Rebecca F, Shirk Megan C, Fall Ray
机构信息
Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.
出版信息
J Bacteriol. 2003 Sep;185(18):5627-31. doi: 10.1128/JB.185.18.5627-5631.2003.
Abstract
Motility on surfaces is an important mechanism for bacterial colonization of new environments. In this report, we describe detection of rapid surface motility in the wild-type Bacillus subtilis Marburg strain, but not in several B. subtilis 168 derivatives. Motility involved formation of rapidly spreading dendritic structures, followed by profuse surface colonies if sufficient potassium ion was present. Potassium ion stimulated surfactin secretion, and the role of surfactin in surface motility was confirmed by deletion of a surfactin synthase gene. Significantly, this motility was independent of flagella. These results demonstrate that wild-type B. subtilis strains can use both swimming and sliding-type mechanisms to move across surfaces.
摘要
表面运动是细菌在新环境中定殖的重要机制。在本报告中，我们描述了在野生型枯草芽孢杆菌马尔堡菌株中检测到快速表面运动，但在几种枯草芽孢杆菌168衍生物中未检测到。运动涉及快速扩散的树枝状结构的形成，如果存在足够的钾离子，则随后会形成大量表面菌落。钾离子刺激表面活性素的分泌，通过缺失表面活性素合成酶基因证实了表面活性素在表面运动中的作用。值得注意的是，这种运动与鞭毛无关。这些结果表明，野生型枯草芽孢杆菌菌株可以利用游动和滑动两种机制在表面移动。
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