Beam S L, Rassnick K M, Moore A S, McDonough S P
Department of Clinical Sciences, Box 31, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Vet Pathol. 2003 Sep;40(5):496-500. doi: 10.1354/vp.40-5-496.
Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins and exist as two isoforms, COX-1 and COX-2. COX-2 is a potent inducible mediator of inflammation. COX-2 is also upregulated in several human tumors and in canine squamous cell, renal cell, and transitional cell carcinomas, prostatic adenocarcinoma, and intestinal neoplasia. The purpose of this study was to determine whether COX-2 is expressed in various feline tumors. Results of this study may help determine whether COX-2 is a potential target for therapeutic and preventive strategies in cats. Immunohistochemical studies were performed on paraffin-embedded tissues using the amplified streptavidin-biotin-horseradish peroxidase system. COX-2 was found in 7 of 19 (37%) feline transitional cell carcinomas and in 2 of 21 (9%) feline oral squamous cell carcinomas. No COX-2 immunoreactivity was detected in cutaneous squamous cell carcinomas (6), adenocarcinomas (nine mammary, eight pulmonary, seven intestinal), lymphomas (six nasal, six intestinal), or 10 vaccine-associated sarcomas. The widespread absence of COX-2 expression in most feline neoplasms might suggest that COX-2 inhibitors would have a low potential as anticancer agents.
环氧化酶（COX）催化前列腺素的合成，以两种同工型COX-1和COX-2的形式存在。COX-2是一种强效的炎症诱导介质。COX-2在几种人类肿瘤以及犬鳞状细胞癌、肾细胞癌、移行细胞癌、前列腺腺癌和肠道肿瘤中也上调。本研究的目的是确定COX-2是否在各种猫科动物肿瘤中表达。本研究结果可能有助于确定COX-2是否是猫治疗和预防策略的潜在靶点。使用扩增的链霉亲和素-生物素-辣根过氧化物酶系统对石蜡包埋组织进行免疫组织化学研究。在19例猫移行细胞癌中有7例（37%）检测到COX-2，在21例猫口腔鳞状细胞癌中有2例（9%）检测到COX-2。在皮肤鳞状细胞癌（6例）、腺癌（9例乳腺、8例肺、7例肠道）、淋巴瘤（6例鼻腔、6例肠道）或10例疫苗相关肉瘤中未检测到COX-2免疫反应性。大多数猫科动物肿瘤中普遍缺乏COX-2表达可能表明COX-2抑制剂作为抗癌药物的潜力较低。
