Nasry Walaa Hamed Shaker, Wang Haili, Jones Kathleen, Dirksen Wessel P, Rosol Thomas J, Rodriguez-Lecompte Juan Carlos, Martin Chelsea K
Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Diagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Vet Sci. 2018 Aug 13;5(3):72. doi: 10.3390/vetsci5030072.
Feline oral squamous cell carcinoma (OSCC) is a highly invasive form of cancer in cats. In human OSCC, cluster of differentiation 147 (CD147) contributes to inflammation and tumor invasiveness. CD147 is a potential therapeutic target, but the expression of CD147 in feline OSCC has not been examined. Immunohistochemistry was used to determine if cyclooxygenase 2 (COX-2) and CD147 expression in feline OSCC biopsies was coordinated. Tumor cells were more likely to express COX-2 (22/43 cases or 51%) compared to stroma (8/43 or 19%) and adjacent oral epithelium (9/31 cases or 29%) ( < 0.05). CD147 was also more likely to occur in tumor cells compared to stroma and adjacent mucosa, with 21/43 (49%) of cases having >50% tumor cells with mild or moderate CD147 expression, compared to 9/28 (32%) in adjacent epithelium and only 5/43 (12%) in adjacent stroma ( < 0.05). In feline OSCC cell lines (SCCF1, SCCF2, and SCCF3), CD147 gene expression was more consistently expressed compared to COX-2, which was 60-fold higher in SCCF2 cells compared to SCCF1 cells ( < 0.05). CD147 expression did not correlate with COX-2 expression and prostaglandin E2 (PGE2) secretion, indicating that they may be independently regulated. CD147 potentially represents a novel therapeutic target for the treatment of feline OSCC and further study of CD147 is warranted.
猫口腔鳞状细胞癌（OSCC）是猫类中一种具有高度侵袭性的癌症形式。在人类OSCC中，分化簇147（CD147）会促进炎症和肿瘤侵袭性。CD147是一个潜在的治疗靶点，但尚未对猫OSCC中CD147的表达进行研究。采用免疫组织化学法来确定猫OSCC活检组织中环氧化酶2（COX - 2）和CD147的表达是否具有协同性。与基质（8/43或19%）和相邻口腔上皮（9/31例或29%）相比，肿瘤细胞更有可能表达COX - 2（22/43例或占51%）（P<0.05）。与基质和相邻黏膜相比，CD147也更易出现在肿瘤细胞中，43例中有21例（49%）的肿瘤细胞中有>50%的细胞呈轻度或中度CD147表达，而相邻上皮中为9/28（32%），相邻基质中仅为5/43（12%）（P<0.05）。在猫OSCC细胞系（SCCF1、SCCF2和SCCF3）中，与COX - 2相比，CD147基因表达更为稳定，SCCF2细胞中的COX - 2表达比SCCF1细胞高60倍（P<0.05）。CD147的表达与COX - 2表达及前列腺素E2（PGE2）分泌无关，表明它们可能受到独立调控。CD147可能是治疗猫OSCC的一个新的治疗靶点，因此有必要对CD147进行进一步研究。
