Biondo A W, Ehrhart E J, Sisson D D, Bulmer B J, De Morais H S A, Solter P F
Department of Veterinary Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802, USA.
Vet Pathol. 2003 Sep;40(5):501-6. doi: 10.1354/vp.40-5-501.
Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) are cardiac hormones involved in electrolyte and fluid homeostasis. Our laboratory has investigated the use of ANP and BNP as diagnostic markers of cardiac disease in cats. We hypothesize that the cardiac distribution of ANP and BNP increases in cats with hypertrophic cardiomyopathy (HCM). Accordingly, we evaluated the immunohistochemical distribution of ANP and BNP in hearts of four cats with naturally occurring HCM relative to five healthy controls. Indirect immunoperoxidase was performed with polyclonal immunoglobulin G against feline ANP (1-28) and proBNP (43-56). In control cats, ANP and BNP immunoreactivity was restricted to the atria. Staining for both peptides was most intense adjacent to the endocardial surface. Auricles stained more diffusely than atria for both peptides. The interstitial capillaries and nerve fibers within the heart were positive only for BNP. Atrial immunoreactivity for ANP and BNP was more diffuse and had a less distinctly layered pattern in HCM than in control cats. Ventricular cardiomyocytes of HCM cats were negative for ANP but stained lightly and diffusely for BNP. The capillaries and nerve fibers remained positive for BNP. We conclude that in cats with HCM, the cardiac distribution of ANP and BNP is more diffuse in the atria and that novel expression of BNP in the ventricular cardiomyocytes occurs.
心房利钠肽（ANP）和脑利钠肽（BNP）是参与电解质和液体稳态的心脏激素。我们实验室研究了将ANP和BNP用作猫心脏疾病诊断标志物的用途。我们假设，患有肥厚型心肌病（HCM）的猫中ANP和BNP的心脏分布会增加。因此，我们评估了相对于五只健康对照猫，四只自然发生HCM的猫心脏中ANP和BNP的免疫组织化学分布。使用针对猫ANP（1-28）和proBNP（43-56）的多克隆免疫球蛋白G进行间接免疫过氧化物酶检测。在对照猫中，ANP和BNP免疫反应性仅限于心房。两种肽的染色在靠近心内膜表面处最为强烈。两种肽在耳廓中的染色比在心房中更弥散。心脏内的间质毛细血管和神经纤维仅对BNP呈阳性。与对照猫相比，HCM猫心房中ANP和BNP的免疫反应性更弥散，且分层模式不那么明显。HCM猫的心室心肌细胞对ANP呈阴性，但对BNP染色浅且弥散。毛细血管和神经纤维对BNP仍呈阳性。我们得出结论，在患有HCM的猫中，ANP和BNP的心脏分布在心房中更弥散，并且BNP在心室心肌细胞中出现了新的表达。
