Takahashi Yōko, Omura Satoshi
Kitasato Institute for Life Sciences, Kitasato University, and The Kitasato Institute, Minato-ku, Tokyo 108-8641, Japan.
J Gen Appl Microbiol. 2003 Jun;49(3):141-54. doi: 10.2323/jgam.49.141.
In order to facilitate the discovery of novel bioactive compounds from microorganisms, various techniques for isolation of new actinomycete strains have been attempted. Studies of the vertical distribution of actinomycetes in soil, isolation of actinomycetes from desert soils or fallen leaves, selective isolation of Kitasatospora strains using novobiocin or Actinoplanes strains using the chemotactic method, and the use of gellan gum as a solidifying agent were carried out. We discovered 9 novel bioactive compounds from actinomycete strains isolated under unusual conditions, and proposed two new genera, five new species and one new subspecies.
为了便于从微生物中发现新型生物活性化合物，人们尝试了各种分离新放线菌菌株的技术。开展了土壤中放线菌垂直分布的研究、从沙漠土壤或落叶中分离放线菌、使用新生霉素选择性分离北里孢菌属菌株或采用趋化方法分离游动放线菌属菌株以及使用结冷胶作为固化剂等工作。我们从在异常条件下分离得到的放线菌菌株中发现了9种新型生物活性化合物，并提出了两个新属、五个新种和一个新亚种。
