Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo, 141-0021, Japan.
J Antibiot (Tokyo). 2022 Oct;75(10):535-541. doi: 10.1038/s41429-022-00560-4. Epub 2022 Sep 7.
In the course of our screening program for new anti-methicillin-resistant Staphylococcus aureus antibiotics, four novel antibiotics, termed wychimicins A-D, were isolated from the culture broth of the rare actinomycete Actinocrispum wychmicini strain MI503-AF4. Wychimicins are spirotetronates possessing a macrocyclic 13-membered ring containing trans-decalin and β-D-xylo-hexopyranose moieties connected to C-17 by an O-glycosidic linkage according to MS, NMR and X-ray analyses. In X-ray crystal structure analysis, the Flack constant was 0.10 (11). The stereochemistry of the spirocarbon C-25 was R. Wychimicins had a minimum inhibitory concentration of 0.125-2 µg ml against methicillin-resistant Staphylococcus aureus.
在我们筛选新型抗耐甲氧西林金黄色葡萄球菌抗生素的过程中，从稀有放线菌 Actinocrispum wychmicini 菌株 MI503-AF4 的发酵液中分离出四种新型抗生素，称为 wychimicins A-D。Wychimicins 是一种具有大环 13 元环的螺缩环四烯，其中包含反式十氢化萘和 β-D-木戊吡喃糖部分，根据 MS、NMR 和 X 射线分析，通过 O-糖苷键连接到 C-17。在 X 射线晶体结构分析中，Flack 常数为 0.10（11）。螺缩碳 C-25 的立体化学为 R。Wychimicins 对耐甲氧西林金黄色葡萄球菌的最小抑制浓度为 0.125-2µg·ml-1。
