Arakawa E T, Tuminello P S, Khare B N, Milham M E
Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
Biopolymers. 2003;72(5):391-8. doi: 10.1002/bip.10438.
We measure the complex index of refraction of Erwina herbicola (also known as Enterobacter agglomerans or Pantoea agglomerans) bacteria (ATTC 33243) over the spectral region from 0.190 to 2.50 microm (4000-52,632 cm(-1)). Transmission measurements are made on solid films of E. herbicola and on suspensions of the bacteria in water. These measurements, combined with spectral reflectance and Kramers-Krönig analysis, allow the determination of the real and imaginary parts over the entire wavelength interval. Accurate and consistent results are obtained for this complex and difficult to measure material. This is part of a continuing series of measurements of the optical constants of representative biological materials that are applicable to the development of methods for detection of airborne biological contaminants, where the material under study is used as a surrogate for a pathogenic agent.
我们测量了成团肠杆菌(也称为聚团肠杆菌或成团泛菌)细菌(ATTC 33243)在0.190至2.50微米(4000 - 52,632厘米⁻¹)光谱范围内的复折射率。对成团肠杆菌的固体薄膜以及细菌在水中的悬浮液进行了透射测量。这些测量结果与光谱反射率和克喇末 - 克朗尼格分析相结合,能够确定整个波长区间的实部和虚部。对于这种复杂且难以测量的材料,我们获得了准确且一致的结果。这是一系列持续测量代表性生物材料光学常数的一部分,这些测量适用于开发检测空气中生物污染物的方法,其中所研究的材料用作病原体的替代物。