Lee Tiger T T, Wang Miki M C, Hou Rolis C W, Chen Liang-Jwu, Su Ruey-Chih, Wang Chang-Sheng, Tzen Jason T C
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227, ROC.
Biosci Biotechnol Biochem. 2003 Aug;67(8):1699-705. doi: 10.1271/bbb.67.1699.
A chimeric gene encoding a precursor polypeptide of sesame 2S albumin, a sulfur-rich seed storage protein, was expressed in transgenic rice plants under the control of the glutelin promoter with the aim of improving the nutritive value of rice. Rice grains harvested from the first generation of ten different transformed lines inherited the transgene, and the accumulated sesame 2S albumin was presumably processed correctly as its mature form in sesame seed. This transgene was specifically expressed in maturing rice seeds with its encoded sesame 2S albumin exclusively accumulated in the seeds. The crude protein content in rice grains from five putative homozygous lines was increased by 0.64-3.54%, and the methionine and cysteine contents of these transgenic rice grains were respectively elevated by 29-76% and 31-75% compared with those of wild-type rice grains.
为提高水稻营养价值，一个编码芝麻2S白蛋白（一种富含硫的种子贮藏蛋白）前体多肽的嵌合基因在谷蛋白启动子的控制下在转基因水稻植株中表达。从十个不同转化株系的第一代收获的水稻种子继承了转基因，并且积累的芝麻2S白蛋白可能在芝麻种子中正确加工成其成熟形式。该转基因在成熟水稻种子中特异性表达，其编码的芝麻2S白蛋白仅在种子中积累。与野生型水稻种子相比，五个推定纯合株系的水稻种子粗蛋白含量提高了0.64 - 3.54%，这些转基因水稻种子的蛋氨酸和半胱氨酸含量分别提高了29 - 76%和31 - 75%。
