Yang Lin, Huang Yu, Wang Han Qing, Chen Zhen-Yu
State Key Laboratory of OXO Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, The Chinese Academy of Sciences of Lanzhou, Lanzhou, China.
J Agric Food Chem. 2003 Sep 10;51(19):5654-60. doi: 10.1021/jf021255u.
The isomeric distribution of conjugated linoleic acids (CLA) in the tissue lipids of hens in relation to that in the diet was examined. Silver-ion high-performance liquid chromatography was used to quantify individual CLA isomers in total tissue lipids, phospholipids, and triacylglycerols. It was found that the deposition of CLA isomers in hen tissues was selective. All tissues including serum, liver, heart, kidney, abdominal fat, and leg and breast muscles had lesser amounts of total cis/trans isomers ranging from 75.87 to 89.13% of total CLA, which was in contrast to the value of 92% of total CLA in the dietary lipids. Total trans/trans isomers in all tissue lipids ranging from 6.11 to 18.02% of total CLA were greater than that in the diet (4.19%). Among the individual trans/trans isomers, all tissues except for adipose tissue and brain incorporated greater amounts of t-12,t-14-18:2, t-11,t-13-18:2,t-10,t-12-18:2, t-9,t-11-18:2, and t-18,t-10-18:2 compared with the values of the diet. Within the cis/trans group, lesser amounts of c-10,t-12/t-10,c-12-18:2 were found to incorporate into all tissues compared with the value of the diet. Serum and liver had higher percentages of c-9,t-11/t-9,c-11, whereas the other tissues had similar levels of this isomer compared with that of the diet. It was also observed that supplementation of CLA in the diet of layer hens decreased the concentration of docosahexaenoic acid (22:6n-3) in all of the tissue lipids. It is concluded that dietary CLA can transfer to the tissue but that incorporation of CLA isomers into the tissue is selective in hens.
研究了母鸡组织脂质中共轭亚油酸（CLA）的异构体分布及其与日粮中CLA异构体分布的关系。采用银离子高效液相色谱法对总组织脂质、磷脂和三酰甘油中的CLA异构体进行定量分析。结果发现，CLA异构体在母鸡组织中的沉积具有选择性。包括血清、肝脏、心脏、肾脏、腹部脂肪、腿部肌肉和胸部肌肉在内的所有组织中，顺式/反式异构体总量占总CLA的比例较低，为75.87%至89.13%，这与日粮脂质中总CLA的92%的值形成对比。所有组织脂质中反式/反式异构体总量占总CLA的比例为6.11%至18.02%，高于日粮中的比例（4.19%）。在单个反式/反式异构体中，除脂肪组织和大脑外，所有组织中t-12,t-14-18:2、t-11,t-13-18:2、t-10,t-12-18:2、t-9,t-11-18:2和t-18,t-10-18:2的含量均高于日粮中的含量。在顺式/反式组中，与日粮相比，所有组织中c-10,t-12/t-10,c-12-18:2的含量较低。血清和肝脏中c-9,t-11/t-9,c-11的百分比更高，而其他组织中该异构体的含量与日粮相似。还观察到，在蛋鸡日粮中添加CLA会降低所有组织脂质中二十二碳六烯酸（22:6n-3）的浓度。研究得出结论，日粮中的CLA可以转移到组织中，但CLA异构体在母鸡组织中的掺入具有选择性。
