Lopes Paula A, Martins Susana V, Pinho Mário S, Alfaia Cristina M, Fontes Carlos M G A, Rodrigues Pedro O, Morais Graça S L, Castro Matilde F, Pinto Rui, Prates José A M
CIISA, Faculdade de Medicina Veterinária, Pólo Universitário do Alto da Ajuda, 1300-477 Lisbon, Portugal.
Nutr Res. 2008 Jul;28(7):480-6. doi: 10.1016/j.nutres.2008.04.002.
Previous reports have demonstrated that conjugated linoleic acid (CLA) acts on body fat accumulation in a variety of animal models. The aim of the present study was to investigate the effect of cis (c)-9,trans (t)-11 and t10,c12 CLA isomers on the number and size of adipocytes from the inguinal and retroperitoneal fats in Wistar male rats. A 5.1% palm oil-based diet was supplemented with CLA isomers as follows: 0.6% of c9,t11, 0.6% of t10,c12, 1.3% of c9,t11 and t10,c12 isomers in mixture, and a control nonsupplemented group for comparative purposes. Fat tissues were prepared on microscope slides for histologic examination using an image-analysis software to count the number of adipocytes and measure cell sizes. The results showed that CLA isomers did not affect (P > .05) either final body and fat depot weights or serum lipids (with the exception of triacylglycerols) and adipocytokines (leptin and adiponectin). Animals fed the c9,t11 CLA isomer diet showed larger adipocytes when compared to other groups. Independently of the CLA dietary treatment, retroperitoneal fat showed larger adipocytes (3319 microm(2)) and therefore a smaller number of adipocytes per unit of area, compared to inguinal fat (3055 microm(2)). Taken together, the data suggest that a palm oil-based diet supplemented with the c9,t11 CLA isomer in Wistar rats, in contrast to the t10,c12 isomer and the mixture of both isomers, increases adipocyte dimensions in inguinal and retroperitoneal fat depots, while having a minor effect in serum lipids and adipocytokines.
先前的报告表明，共轭亚油酸（CLA）在多种动物模型中对体脂积累有作用。本研究的目的是调查顺式（c）-9，反式（t）-11和t10，c12共轭亚油酸异构体对Wistar雄性大鼠腹股沟和腹膜后脂肪中脂肪细胞数量和大小的影响。以5.1%的棕榈油为基础的饮食补充CLA异构体如下：0.6%的c9，t11，0.6%的t10，c12，1.3%的c9，t11和t10，c12异构体混合物，以及一个不补充的对照组用于比较。将脂肪组织制备在载玻片上，使用图像分析软件进行组织学检查，以计数脂肪细胞数量并测量细胞大小。结果表明，CLA异构体对最终体重、脂肪储存重量、血清脂质（除三酰甘油外）和脂肪细胞因子（瘦素和脂联素）均无影响（P>.05）。与其他组相比，喂食c9，t11 CLA异构体饮食的动物脂肪细胞更大。与腹股沟脂肪（3055平方微米）相比，无论CLA饮食处理如何，腹膜后脂肪的脂肪细胞更大（3319平方微米），因此单位面积内的脂肪细胞数量更少。综上所述，数据表明，与t10，c12异构体和两种异构体的混合物相比，在Wistar大鼠中以棕榈油为基础的饮食补充c9，t11 CLA异构体可增加腹股沟和腹膜后脂肪储存中的脂肪细胞尺寸，而对血清脂质和脂肪细胞因子的影响较小。
