Silva Ana Cristina, Filipe Mario, Kuerner Klaus-Michael, Steinbeisser Herbert, Belo Jose António
Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Apartado 14, 2781-901 Oeiras, Portugal.
Development. 2003 Oct;130(20):4943-53. doi: 10.1242/dev.00705.
We analyzed the endogenous requirement for Cerberus in Xenopus head development. 'Knockdown' of Cerberus function by antisense morpholino oligonucleotides did not impair head formation in the embryo. In contrast, targeted increase of BMP, Nodal and Wnt signaling in the anterior dorsal-endoderm (ADE) resulted in synergistic loss of anterior head structures, without affecting more posterior axial ones. Remarkably, those head phenotypes were aggravated by simultaneous depletion of Cerberus. These experiments demonstrated for the first time that endogenous Cerberus protein can inhibit BMP, Nodal and Wnt factors in vivo. Conjugates of dorsal ectoderm (DE) and ADE explants in which Cerberus function was 'knocked down' revealed the requirement of Cerberus in the ADE for the proper induction of anterior neural markers and repression of more posterior ones. This data supports the view that Cerberus function is required in the leading edge of the ADE for correct induction and patterning of the neuroectoderm.
我们分析了非洲爪蟾头部发育过程中内源性Cerberus的需求。通过反义吗啉代寡核苷酸“敲低”Cerberus功能并不会损害胚胎中的头部形成。相反，在前背侧内胚层（ADE）中靶向增加骨形态发生蛋白（BMP）、节点蛋白（Nodal）和Wnt信号传导会导致前头部结构协同缺失，而不影响更靠后的轴向结构。值得注意的是，同时缺失Cerberus会加剧这些头部表型。这些实验首次证明内源性Cerberus蛋白在体内可抑制BMP、Nodal和Wnt因子。在Cerberus功能被“敲低”的背侧外胚层（DE）和ADE外植体的结合物中，揭示了ADE中Cerberus对于正确诱导前神经标记物和抑制更靠后的神经标记物的需求。该数据支持了这样一种观点，即ADE前沿需要Cerberus功能来正确诱导神经外胚层并使其形成模式。
