Crabbe J C, Cotnam C J, Cameron A J, Schlumbohm J P, Rhodes J S, Metten P, Wahlsten D
Portland Alcohol Research Center, Department of Behavioral Neuroscience, Oregon Health & Science University, Portland, Oregon, USA.
Genes Brain Behav. 2003 Aug;2(4):201-13. doi: 10.1034/j.1601-183x.2003.00023.x.
Mice from 8 to 21 inbred strains were tested for sensitivity to ethanol intoxication using a range of doses and three different measures: the screen test, the dowel test and a test of grip strength. Strains differed under nearly all conditions. For the dowel test, two dowel widths were employed, and mice were tested immediately or 30 min after ethanol. For the dowel and screen tests, low doses failed to affect some strains, and the highest doses failed to discriminate among mice, maximally affecting nearly all. For grip strength, a single ethanol dose was used, and mice of all strains were affected. Pharmacokinetic differences among strains were significant, but these could not account for strain differences in intoxication. For doses and test conditions in the middle range, there were only modest correlations among strain means within a test. In addition, genotypic correlations across tests were modest to quite low. These results suggest that different specific versions of a test reflect the influence of different genes, and that genetic influences on different tests were also distinct.
对8至21个近交系小鼠进行了乙醇中毒敏感性测试，使用了一系列剂量和三种不同的测量方法：筛检试验、暗榫试验和握力测试。在几乎所有条件下，各品系均存在差异。对于暗榫试验，使用了两种暗榫宽度，小鼠在乙醇处理后立即或30分钟后进行测试。对于暗榫试验和筛检试验，低剂量未能影响某些品系，而高剂量未能区分小鼠，几乎对所有小鼠都产生了最大影响。对于握力测试，使用了单一乙醇剂量，所有品系的小鼠均受到影响。品系间的药代动力学差异显著，但这些差异无法解释中毒方面的品系差异。对于中等剂量范围和测试条件，同一测试中品系均值之间的相关性仅为中等。此外，不同测试之间的基因型相关性从中等到非常低。这些结果表明，测试的不同特定版本反映了不同基因的影响，并且基因对不同测试的影响也是不同的。
