Steven F S, Griffin M M, Cederholm-Williams S A, Mangel W F, Maier H
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Anticancer Res. 1991 Mar-Apr;11(2):641-7.
Tumour cells possess a cell surface protease referred to as guanidinobenzoatase (GB). The active centre of GB binds the fluorescent probe 9-amino acridine (9-AA) and this binding enables cells possessing active GB to be located by fluorescent microscopy. GB binding of 9-AA was inhibited by prior treatment of sections of tumour tissue with a specific polyclonal antibody recognising the tumour associated protease tissue plasminogen activator (t-PA). GB binding of 9-AA was also inhibited by prior treatment of sections of tumour tissues with PAI-I, a protein inhibitor of plasminogen activatory. We conclude from these studies and kinetic analyses that GB and t-PA are very similar both in structure and function.
肿瘤细胞具有一种称为胍基苯甲酸酶（GB）的细胞表面蛋白酶。GB的活性中心与荧光探针9-氨基吖啶（9-AA）结合，这种结合使得具有活性GB的细胞能够通过荧光显微镜进行定位。用识别肿瘤相关蛋白酶组织纤溶酶原激活物（t-PA）的特异性多克隆抗体预先处理肿瘤组织切片，可抑制GB与9-AA的结合。用纤溶酶原激活物的蛋白抑制剂PAI-1预先处理肿瘤组织切片，也可抑制GB与9-AA的结合。我们从这些研究和动力学分析得出结论，GB和t-PA在结构和功能上非常相似。
