Krastanov Albert, Yoshida Toshiomi
Department of Biotechnology, University of Food Technologies, 4002 Plovdiv, Bulgaria.
J Ind Microbiol Biotechnol. 2003 Oct;30(10):593-8. doi: 10.1007/s10295-003-0087-4. Epub 2003 Aug 30.
In recent decades, the production of palatinose has aroused great interest since this structural isomer of sucrose has interesting potential. We describe a simple and effective method of immobilizing Serratia plymuthica cells in chitosan. The sucrose isomerase activity of immobilized preparations was enhanced many times by activation with fresh nutrient medium and subsequent drying. The preparations obtained were physically very stable with high enzyme activity and excellent operational stability. The effect of temperature, pH and substrate concentration on enzyme activity of the immobilized cells was investigated. Using immobilized cells, a complete conversion of sucrose (40% solution) into palatinose was achieved in 4 h in a "batch"-type enzyme reactor. The use of free or immobilized cells had no effect on the composition of the solution, in particular the sugar content. The palatinose content was 80% and that of trehalulose 7%.
近几十年来，帕拉金糖的生产引起了极大的兴趣，因为这种蔗糖的结构异构体具有有趣的潜力。我们描述了一种将粘质沙雷氏菌细胞固定在壳聚糖中的简单有效方法。通过用新鲜营养培养基激活并随后干燥，固定化制剂的蔗糖异构酶活性提高了许多倍。所获得的制剂在物理上非常稳定，具有高酶活性和出色的操作稳定性。研究了温度、pH值和底物浓度对固定化细胞酶活性的影响。在“分批”式酶反应器中，使用固定化细胞在4小时内实现了蔗糖（40%溶液）向帕拉金糖的完全转化。使用游离或固定化细胞对溶液的组成没有影响，特别是糖含量。帕拉金糖含量为80%，海藻酮含量为7%。
