de Gandarias J M, Irazusta J, Echevarría E, San Martín E, Casis L
Department of Physiology, Medical School, University of the Basque Country, Leioa, Spain.
Int J Dev Biol. 1992 Dec;36(4):583-5.
Brain aminopeptidase activity has been suggested as a candidate for the regulation and biotransformation of several neuropeptides. In this paper, changes in Lys- and Leu-aminopeptidase activities in rat brain hemispheres, cerebellum and medulla were examined in 1-, 3-, 5- and 7-days postnatal subjects. Aminopeptidase activities were studied by measuring the rate of hydrolysis of the artificial chromogenic substrates Lys- and Leu-2-naphthylamides (fluorimetrically detected in triplicate). Both enzyme activities show a decrease on the 3rd day of life followed by increases on the 5th and 7th day postbirth. It is suggested that these activities could play a part in the neurochemical changes that take place during axonal and dendritic growth in the rat brain.
脑氨肽酶活性被认为是几种神经肽调节和生物转化的一个候选因素。在本文中，对出生后1天、3天、5天和7天的大鼠脑半球、小脑和延髓中赖氨酸和亮氨酸氨肽酶活性的变化进行了检测。通过测量人工生色底物赖氨酸和亮氨酸-2-萘酰胺的水解速率（荧光法一式三份检测）来研究氨肽酶活性。两种酶活性在出生后第3天均下降，随后在出生后第5天和第7天升高。提示这些活性可能参与了大鼠脑轴突和树突生长过程中发生的神经化学变化。
