De Gandarias J M, Irazusta J, Echevarria E, Mugica J, Casis L
Department of Physiology, University of the Basque Country, Leioa, Spain.
Int J Dev Biol. 1989 Dec;33(4):491-3.
Research concerning the functional role of brain peptides is performed, in part, by studying peptidase enzymes which might be involved in brain peptide processing or inactivation. Aminopeptidase (AP) activity has been proposed as a candidate regulator of the degradation of these peptides. In this paper, changes in Lys- and Leu-aminopeptidase activities in rat brain hemispheres, cerebellum and medulla were examined in 20 day fetuses and one day postnatal subjects. Aminopeptidase activities were studied by measuring the rate of hydrolysis of the artificial substrates Lys- and Leu-2-naphthylamides (fluorimetrically detected in triplicate). Both enzyme activities increase from the last fetal stage up to the first day of birth in all the brain areas examined except for the case of Leu-AP activity in the medulla. It is suggested that these activities play a part in the neurochemical changes that take place during rat brain maturation, possibly by regulating the activity of several neuroactive peptides.
关于脑肽功能作用的研究，部分是通过研究可能参与脑肽加工或失活的肽酶来进行的。氨肽酶（AP）活性已被认为是这些肽降解的候选调节因子。本文研究了20日龄胎儿和出生后1天的大鼠脑半球、小脑和延髓中赖氨酸和亮氨酸氨肽酶活性的变化。通过测量人工底物赖氨酸和亮氨酸 - 2 - 萘酰胺的水解速率（一式三份荧光检测）来研究氨肽酶活性。除延髓中亮氨酸 - AP活性外，在所检查的所有脑区中，两种酶活性从胎儿末期到出生第一天均增加。有人认为，这些活性可能通过调节几种神经活性肽的活性，在大鼠脑成熟过程中发生的神经化学变化中发挥作用。
