Martínez-Millán L, de Gandarias J M, Irazusta J, Echevarría E, Casis L
Department of Physiology, Medical School, University of the Basque Country, Bilbao, Spain.
Int J Dev Neurosci. 1993 Feb;11(1):11-5. doi: 10.1016/0736-5748(93)90030-h.
Aminopeptidase activity has been proposed as a candidate regulator of the degradation of several neuroactive peptides. In this paper, possible changes in Lys-, Arg-, Asp- and Tyr-aminopeptidase activities in the cat brain neocortex (frontal, parietal, area 17 and areas 18 and 19 as a whole) were examined during two of the first stages of the critical developmental period (15 and 30 days postbirth). Aminopeptidase activities were studied by measuring the rate of hydrolysis of the artificial substrates Lys-, Arg-, Asp- and Tyr-2-naphthylamides (fluorimetrically detected in triplicate). Lys- and Arg-aminopeptidase activities do not show significant changes between the first the second stage in any of the cortical regions assayed. On the contrary, in all the cortical structures, activity with Tyr-2-naphthylamide shows significant increase with age. In contrast, the activity of Asp-aminopeptidase shows significant decreases on the 30th day (except in area 17). It is suggested that these activities play a part in the neurochemical changes that take place during cat brain maturation.
氨肽酶活性被认为是几种神经活性肽降解的候选调节因子。本文研究了在关键发育期的前两个阶段（出生后15天和30天）猫脑新皮质（额叶、顶叶、17区以及整个18区和19区）中赖氨酸、精氨酸、天冬氨酸和酪氨酸氨肽酶活性的可能变化。通过测量人工底物赖氨酸、精氨酸、天冬氨酸和酪氨酸 - 2 - 萘酰胺的水解速率来研究氨肽酶活性（以荧光法一式三份检测）。在所检测的任何皮质区域中，赖氨酸和精氨酸氨肽酶活性在第一阶段和第二阶段之间均未显示出显著变化。相反，在所有皮质结构中，酪氨酸 - 2 - 萘酰胺的活性随年龄显著增加。相比之下，天冬氨酸氨肽酶的活性在第30天显著降低（17区除外）。提示这些活性在猫脑成熟过程中发生的神经化学变化中起作用。
