Alves Sandra, Kalberer Markus, Zenobi Renato
Laboratory for Organic Chemistry, Swiss Federal Institute of Technology, CH-8093 Zürich, Switzerland.
Rapid Commun Mass Spectrom. 2003;17(18):2034-8. doi: 10.1002/rcm.1149.
We report the detection of nanoparticles formed by irradiating matrix-assisted laser desorption/ionization (MALDI) matrix samples. This is direct evidence for the ejection of large size aggregates in the MALDI process. Nanometer-size particles were generated via a tunable solid-state UV laser, irradiating a sample placed in a nitrogen atmosphere. Size distribution measurements were performed using a differential mobility analyzer and a condensation particle counter. Particles in the 10-1000 nm size range were detected. The dependence of the particle size distribution on the laser fluence, wavelength and matrix was investigated. The observed effects are discussed and related to the MALDI ablation dynamics and gas-phase processes.
我们报告了通过辐照基质辅助激光解吸/电离（MALDI）基质样品而形成的纳米颗粒的检测情况。这是MALDI过程中喷出大尺寸聚集体的直接证据。通过可调谐固态紫外激光辐照置于氮气气氛中的样品来生成纳米尺寸的颗粒。使用差分迁移率分析仪和冷凝粒子计数器进行尺寸分布测量。检测到尺寸范围在10 - 1000 nm的颗粒。研究了颗粒尺寸分布对激光能量密度、波长和基质的依赖性。对观察到的效应进行了讨论，并将其与MALDI烧蚀动力学和气相过程相关联。
