Wichmann J M, Lupulescu C, Wöste L, Lindinger A
Freie Universität Berlin, Institut für Experimentalphysik, Arnimallee 14, 14195 Berlin, Germany.
Rapid Commun Mass Spectrom. 2009 Apr;23(8):1105-8. doi: 10.1002/rcm.3974.
We observe a substantial matrix-assisted laser desorption/ionization (MALDI) signal when irradiating femtosecond laser pulses in the near-infrared spectral range centered around 800 nm and using standard MALDI matrices with absorption bands in the ultraviolet (UV) regime. The laser pulse energy dependence of this novel phenomenon is investigated in comparison with MALDI with near-UV laser pulses. Our observations show that multiphoton absorption/ionization could be a major issue among the MALDI processes when the sample is irradiated with ultra-short laser pulses.
当在以800nm为中心的近红外光谱范围内照射飞秒激光脉冲，并使用在紫外（UV）波段具有吸收带的标准基质辅助激光解吸/电离（MALDI）基质时，我们观察到了强烈的MALDI信号。与近紫外激光脉冲的MALDI相比，研究了这种新现象的激光脉冲能量依赖性。我们的观察结果表明，当用超短激光脉冲照射样品时，多光子吸收/电离可能是MALDI过程中的一个主要问题。
