Flores Pérez Carmen, Juárez Olguín Hugo, Flores Pérez Janett, Toledo López Alejandra, Lares Asseff Ismael, Alvarez García Carlos
Department of Pharmacology, Instituto Nacional de Pediatría, CP 04530 Mexico City, Mexico.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Sep 25;795(1):141-4. doi: 10.1016/s1570-0232(03)00474-4.
The aim of the present study was to develop a simple method to measure ranitidine, using 100 microL of plasma, by high-performance liquid chromatography with a Symmetry C(18) column and UV detection at 313 nm. Linearity was assessed in the range from 50 to 1500 ng ml(-1) and had a correlation coefficient of 0.999. The inter- and intra-day coefficients of variation were less than 7%. The limits of detection and quantitation were 5 and 15 ng ml(-1), respectively. Drug levels were determined satisfactorily in three patients. A simple and reliable method was developed which uses a microvolume of plasma, particularly useful in low-weight children.
