Lee Sang Ah, Kang Daehee, Seo Sang Soo, Jeong Jeongmi Kim, Yoo Keun Young, Jeon Yong Tark, Kim Jae Weon, Park Noh Hyun, Kang Soon Beom, Lee Hyo Pyo, Song Yong Sang
Department of Preventive Medicine, College of Medicine, Seoul National University, 28 Yongun-dong, Chongno-gu, Seoul 110-799, South Korea.
Cancer Lett. 2003 Aug 20;198(2):187-92. doi: 10.1016/s0304-3835(03)00312-4.
This study determined the distribution of high-risk HPV type infection in cervical cancer using newly developed oligonucleotide chips (HPVDNAChips). The study subjects included 80 cases of cervical neoplasia and 746 controls with a normal Pap smear. For HPV genotyping, the commercially available HPVDNAChips was used. The risk of cervical cancer was increased in women with a family history of cervical cancer (adjusted OR=2.3, 95% CI: 0.92-6.17) and in smokers (adjusted OR=3.2, 95% CI: 1.45-7.06). There was also a trend of increased risk with the number of full term pregnancies (P(for trend)<0.001). There were only 7.2% (54 of 746) infected high-risk HPV types in the control, whereas 54.5% (six of 11) and 76.5% (52 of 68) were infected in the CIN and cervical cancer, respectively. Multiple HPV infection was observed in 0.5% (three of 592) of the control group but in 9.1% (seven of 77) of cases. Multivariate analysis revealed that subjects infected with multiple HPV types had a 31.8-fold (95% CI: 7.50-134.75) higher risk of cervical cancer, while the single HPV type had a 19.9-fold increased risk (95% CI: 10.90-36.18) (P(for trend)<0.001). These results show that the detection and typing of HPV infection by HPVDNAChip can be a useful in clinical applications because it provides information on multiple infections and the types of HPV in addition to HPV infection status.
本研究采用新开发的寡核苷酸芯片（HPV DNA芯片）确定宫颈癌中高危型HPV感染的分布情况。研究对象包括80例宫颈肿瘤患者和746例巴氏涂片正常的对照者。对于HPV基因分型，使用了市售的HPV DNA芯片。有宫颈癌家族史的女性（校正比值比=2.3，95%可信区间：0.92 - 6.17）和吸烟者（校正比值比=3.2，95%可信区间：1.45 - 7.06）患宫颈癌的风险增加。随着足月妊娠次数的增加，风险也有增加趋势（趋势检验P<0.001）。对照组中只有7.2%（746例中的54例）感染了高危型HPV，而在CIN和宫颈癌中分别为54.5%（11例中的6例）和76.5%（68例中的52例）。在对照组的0.5%（592例中的3例）中观察到多重HPV感染，但在病例组中为9.1%（77例中的7例）。多变量分析显示，感染多种HPV类型的受试者患宫颈癌的风险高31.8倍（95%可信区间：7.50 - 134.75），而单一HPV类型的风险增加19.9倍（95%可信区间：10.90 - 36.18）（趋势检验P<0.001）。这些结果表明，通过HPV DNA芯片检测HPV感染及其分型在临床应用中可能有用，因为它除了提供HPV感染状态外，还提供了多重感染和HPV类型的信息。
