Karlsson L M, Tengvall P, Lundström I, Arwin H
Department of Physics and Measurement Technology, Linköping University, SE-58183 Linköping, Sweden.
J Colloid Interface Sci. 2003 Oct 1;266(1):40-7. doi: 10.1016/s0021-9797(03)00595-2.
Human serum albumin was adsorbed into porous silicon layers with thickness up to 3 microm and with different mean pore radius in the range 4.5-10 nm. The adsorbed amount of protein was quantified by I(125) radioactive labeling techniques and ellipsometry. The results show that albumin penetrated into the pores when the mean pore radius was larger than 5.5 nm, but could not totally occupy the available surface area when the layer thickness was larger than 1 microm. Loading of albumin both into porous layers and onto plane silicon as a function of albumin concentration was also investigated. These measurements show that loading of protein increased with protein concentration at least up to 10 mg/ml for porous silicon and up to 1 mg/ml for plane silicon. The maximum deposition into the type of porous layers used here was 28 microg/cm(2), compared to 0.36 microg/cm(2) for plane silicon.
人血清白蛋白被吸附到厚度达3微米、平均孔径在4.5 - 10纳米范围内的多孔硅层中。蛋白质的吸附量通过I(125)放射性标记技术和椭偏仪进行定量。结果表明，当平均孔径大于5.5纳米时，白蛋白会渗透到孔中，但当层厚度大于1微米时，白蛋白不能完全占据可用表面积。还研究了白蛋白在多孔层和平面硅上的负载量随白蛋白浓度的变化。这些测量结果表明，对于多孔硅，蛋白质负载量随蛋白质浓度增加，至少在蛋白质浓度达到10毫克/毫升时如此，对于平面硅，至少在蛋白质浓度达到1毫克/毫升时如此递增。与平面硅的0.36微克/平方厘米相比，此处所用类型的多孔层中的最大沉积量为28微克/平方厘米。
