Institute of Quantum Beam Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki, 310-8512, Japan.
Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, New South Wales, 2234, Australia.
Anal Sci. 2020 Nov 10;36(11):1331-1335. doi: 10.2116/analsci.20P160. Epub 2020 Jun 12.
In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (XRR) and NR. The Si disc with MAO film was applied for an in-situ NR experiment, and its NR profiles before/after adsorption of GOD were continuously measured with a flow cell. The results indicated that the negatively-charged GOD molecules hardly penetrate into the narrow pore channel (pore diameter = ca. 10 nm) with opposite surface charge.
在本研究中，采用原位中子反射测量法（NR）研究了葡萄糖氧化酶（GOD）在介孔氧化铝（MAO）薄膜上的吸附。MAO 薄膜沉积在盖玻片和硅片上，通过 X 射线反射法（XRR）和 NR 对其孔结构进行了表征。带有 MAO 薄膜的硅片用于原位 NR 实验，并通过流池连续测量 GOD 吸附前后的 NR 谱。结果表明，带负电荷的 GOD 分子由于表面带相反电荷而很难进入孔径约为 10nm 的窄孔道。
